Construct: shRNA TRCN0000015736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006354.2-927s1c1
DNA Barcode:
CAGCCCAAGATCCAGGAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TADA3 (10474)
Gene Description:
transcriptional adaptor 3
Transcript:
RefSeq NM_006354.2 (NON-CURRENT)
Match location:
Position 927 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCCCAAGATCCAGGAATAT
Hairpin Sequence:
5'-CCGG-CAGCCCAAGATCCAGGAATAT-CTCGAG-ATATTCCTGGATCTTGGGCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCCCAAGATCCAGGAATATCTCGAGATATTCCTGGATCTTGGGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCCCAAGATCCAGGAATATCTCGAGATATTCCTGGATCTTGGGCTG-3'

Other clones with same target sequence:

  1. TRCN0000276449