Construct: shRNA TRCN0000015788

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173631.2-1276s1c1
DNA Barcode:
CCTTACAAAGTCCCACCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF547 (284306)
Gene Description:
zinc finger protein 547
Transcript:
RefSeq NM_173631.2 (NON-CURRENT)
Match location:
Position 1276 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015788)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTACAAAGTCCCACCTCAT
Hairpin Sequence:
5'-CCGG-CCTTACAAAGTCCCACCTCAT-CTCGAG-ATGAGGTGGGACTTTGTAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTACAAAGTCCCACCTCATCTCGAGATGAGGTGGGACTTTGTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTACAAAGTCCCACCTCATCTCGAGATGAGGTGGGACTTTGTAAGG-3'

Other clones with same target sequence:

(none)