Construct: shRNA TRCN0000015850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144689.3-595s1c1
DNA Barcode:
CTCAACATTCAAGATGTCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF420 (147923)
Gene Description:
zinc finger protein 420
Transcript:
RefSeq NM_144689.3 (NON-CURRENT)
Match location:
Position 595 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAACATTCAAGATGTCATT
Hairpin Sequence:
5'-CCGG-CTCAACATTCAAGATGTCATT-CTCGAG-AATGACATCTTGAATGTTGAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAACATTCAAGATGTCATTCTCGAGAATGACATCTTGAATGTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAACATTCAAGATGTCATTCTCGAGAATGACATCTTGAATGTTGAG-3'

Other clones with same target sequence:

(none)