Construct: shRNA TRCN0000015868

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018952.3-1466s1c1
DNA Barcode:
GCACATACTACCTAGAAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HOXB6 (3216)
Gene Description:
homeobox B6
Transcript:
RefSeq NM_018952.3 (NON-CURRENT)
Match location:
Position 1466 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015868)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATACTACCTAGAAGTAA
Hairpin Sequence:
5'-CCGG-GCACATACTACCTAGAAGTAA-CTCGAG-TTACTTCTAGGTAGTATGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATACTACCTAGAAGTAACTCGAGTTACTTCTAGGTAGTATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATACTACCTAGAAGTAACTCGAGTTACTTCTAGGTAGTATGTGC-3'

Other clones with same target sequence:

(none)