Construct: shRNA TRCN0000016039

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007375.3-666s1c1
DNA Barcode:
CCTAATTCTAAGCAAAGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TARDBP (23435)
Gene Description:
TAR DNA binding protein
Transcript:
RefSeq NM_007375.3 (CURRENT)
Match location:
Position 666 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016039)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAATTCTAAGCAAAGCCAA
Hairpin Sequence:
5'-CCGG-CCTAATTCTAAGCAAAGCCAA-CTCGAG-TTGGCTTTGCTTAGAATTAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAATTCTAAGCAAAGCCAACTCGAGTTGGCTTTGCTTAGAATTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAATTCTAAGCAAAGCCAACTCGAGTTGGCTTTGCTTAGAATTAGG-3'

Other clones with same target sequence:

(none)