Construct: shRNA TRCN0000016138

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005253.3-1943s1c1
DNA Barcode:
GCAGTGAGTATTGGAAGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOSL2 (2355)
Gene Description:
FOS like 2, AP-1 transcription factor subunit
Transcript:
RefSeq NM_005253.3 (NON-CURRENT)
Match location:
Position 1943 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016138)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGAGTATTGGAAGACTT
Hairpin Sequence:
5'-CCGG-GCAGTGAGTATTGGAAGACTT-CTCGAG-AAGTCTTCCAATACTCACTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGAGTATTGGAAGACTTCTCGAGAAGTCTTCCAATACTCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGAGTATTGGAAGACTTCTCGAGAAGTCTTCCAATACTCACTGC-3'

Other clones with same target sequence:

  1. TRCN0000329710