Construct: shRNA TRCN0000016151

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016423.1-611s1c1
DNA Barcode:
GCTTCAACTCTATCCTTGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF219 (51222)
Gene Description:
zinc finger protein 219
Transcript:
RefSeq NM_016423.1 (NON-CURRENT)
Match location:
Position 611 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016151)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCAACTCTATCCTTGCTT
Hairpin Sequence:
5'-CCGG-GCTTCAACTCTATCCTTGCTT-CTCGAG-AAGCAAGGATAGAGTTGAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCAACTCTATCCTTGCTTCTCGAGAAGCAAGGATAGAGTTGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCAACTCTATCCTTGCTTCTCGAGAAGCAAGGATAGAGTTGAAGC-3'

Other clones with same target sequence:

(none)