Construct: shRNA TRCN0000016202

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199005.1-1154s1c1
DNA Barcode:
CAGAGGAGCTACACTATAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF322P1 (387328)
Gene Description:
zinc finger protein 322 pseudogene 1
Transcript:
RefSeq NM_199005.1 (NON-CURRENT)
Match location:
Position 1154 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016202)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGGAGCTACACTATAAAT
Hairpin Sequence:
5'-CCGG-CAGAGGAGCTACACTATAAAT-CTCGAG-ATTTATAGTGTAGCTCCTCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGGAGCTACACTATAAATCTCGAGATTTATAGTGTAGCTCCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGGAGCTACACTATAAATCTCGAGATTTATAGTGTAGCTCCTCTG-3'

Other clones with same target sequence:

(none)