Construct: shRNA TRCN0000016326

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_373255.2-96s1c1
DNA Barcode:
GCTCAACCAAACTGCAGCGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC392228 (392228)
Gene Description:
similar to hypothetical class II basic helix-loop-helix protein
Transcript:
RefSeq XM_373255.2 (NON-CURRENT)
Match location:
Position 96 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016326)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAACCAAACTGCAGCGTT
Hairpin Sequence:
5'-CCGG-GCTCAACCAAACTGCAGCGTT-CTCGAG-AACGCTGCAGTTTGGTTGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAACCAAACTGCAGCGTTCTCGAGAACGCTGCAGTTTGGTTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAACCAAACTGCAGCGTTCTCGAGAACGCTGCAGTTTGGTTGAGC-3'

Other clones with same target sequence:

(none)