Construct: shRNA TRCN0000016434

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133267.1-968s1c1
DNA Barcode:
GAGAGAATTCTCTTCCAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSX2 (170825)
Gene Description:
GS homeobox 2
Transcript:
RefSeq NM_133267.1 (NON-CURRENT)
Match location:
Position 968 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016434)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGAATTCTCTTCCAACAT
Hairpin Sequence:
5'-CCGG-GAGAGAATTCTCTTCCAACAT-CTCGAG-ATGTTGGAAGAGAATTCTCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGAATTCTCTTCCAACATCTCGAGATGTTGGAAGAGAATTCTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGAATTCTCTTCCAACATCTCGAGATGTTGGAAGAGAATTCTCTC-3'

Other clones with same target sequence:

(none)