Construct: shRNA TRCN0000016460

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_038567.8-1490s1c1
DNA Barcode:
GCTGAGAGTAAACTGAAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTA3 (57504)
Gene Description:
metastasis associated 1 family member 3
Transcript:
RefSeq XM_038567.8 (NON-CURRENT)
Match location:
Position 1490 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016460)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGAGTAAACTGAAACAA
Hairpin Sequence:
5'-CCGG-GCTGAGAGTAAACTGAAACAA-CTCGAG-TTGTTTCAGTTTACTCTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGAGTAAACTGAAACAACTCGAGTTGTTTCAGTTTACTCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGAGTAAACTGAAACAACTCGAGTTGTTTCAGTTTACTCTCAGC-3'

Other clones with same target sequence:

(none)