Construct: shRNA TRCN0000016508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_370738.1-3137s1c1
DNA Barcode:
CCGTCACAATTTCCCTCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHD8 (57680)
Gene Description:
chromodomain helicase DNA binding protein 8
Transcript:
RefSeq XM_370738.1 (NON-CURRENT)
Match location:
Position 3137 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTCACAATTTCCCTCAGAA
Hairpin Sequence:
5'-CCGG-CCGTCACAATTTCCCTCAGAA-CTCGAG-TTCTGAGGGAAATTGTGACGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTCACAATTTCCCTCAGAACTCGAGTTCTGAGGGAAATTGTGACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTCACAATTTCCCTCAGAACTCGAGTTCTGAGGGAAATTGTGACGG-3'

Other clones with same target sequence:

(none)