Construct: shRNA TRCN0000016583
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_138330.1-1242s1c1
- DNA Barcode:
- CGATCCTCAAATCTTACGGAA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- ZNF675 (171392)
- Gene Description:
- zinc finger protein 675
- Transcript:
- RefSeq NM_138330.1 (NON-CURRENT)
- Match location:
- Position 1242 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Table Loading...
Sequence Information
- Target Sequence:
- CGATCCTCAAATCTTACGGAA
- Hairpin Sequence:
- 5'-CCGG-CGATCCTCAAATCTTACGGAA-CTCGAG-TTCCGTAAGATTTGAGGATCG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGATCCTCAAATCTTACGGAACTCGAGTTCCGTAAGATTTGAGGATCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGATCCTCAAATCTTACGGAACTCGAGTTCCGTAAGATTTGAGGATCG-3'