Construct: shRNA TRCN0000016585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138330.1-329s1c1
DNA Barcode:
CCCAGTAATGTGTTCTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF675 (171392)
Gene Description:
zinc finger protein 675
Transcript:
RefSeq NM_138330.1 (NON-CURRENT)
Match location:
Position 329 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTAATGTGTTCTCATTT
Hairpin Sequence:
5'-CCGG-CCCAGTAATGTGTTCTCATTT-CTCGAG-AAATGAGAACACATTACTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTAATGTGTTCTCATTTCTCGAGAAATGAGAACACATTACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTAATGTGTTCTCATTTCTCGAGAAATGAGAACACATTACTGGG-3'

Other clones with same target sequence:

(none)