Construct: shRNA TRCN0000016593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001717.2-969s1c1
DNA Barcode:
CCACACCATTTCAGGTTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BNC1 (646)
Gene Description:
basonuclin 1
Transcript:
RefSeq NM_001717.2 (NON-CURRENT)
Match location:
Position 969 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACACCATTTCAGGTTGAAA
Hairpin Sequence:
5'-CCGG-CCACACCATTTCAGGTTGAAA-CTCGAG-TTTCAACCTGAAATGGTGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACACCATTTCAGGTTGAAACTCGAGTTTCAACCTGAAATGGTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACACCATTTCAGGTTGAAACTCGAGTTTCAACCTGAAATGGTGTGG-3'

Other clones with same target sequence:

(none)