Construct: shRNA TRCN0000016625

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_377877.1-396s1c1
DNA Barcode:
GCTCTTTGTTGGAGAGGTGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC402200 (402200)
Gene Description:
similar to Transcription initiation factor TFIID subunit 11 (Transcription initiation factor TFIID 28 kDa subunit) (TAF(II)28) (TAFII-28) (TAFII28) (TFIID subunit p30-beta)
Transcript:
RefSeq XM_377877.1 (NON-CURRENT)
Match location:
Position 396 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016625)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTTTGTTGGAGAGGTGGT
Hairpin Sequence:
5'-CCGG-GCTCTTTGTTGGAGAGGTGGT-CTCGAG-ACCACCTCTCCAACAAAGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTTTGTTGGAGAGGTGGTCTCGAGACCACCTCTCCAACAAAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTTTGTTGGAGAGGTGGTCTCGAGACCACCTCTCCAACAAAGAGC-3'

Other clones with same target sequence:

(none)