Construct: shRNA TRCN0000016646

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001755.1-524s1c1
DNA Barcode:
GCTGGCAGTAACTGGCAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBFB (865)
Gene Description:
core-binding factor subunit beta
Transcript:
RefSeq NM_001755.1 (NON-CURRENT)
Match location:
Position 524 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016646)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGCAGTAACTGGCAAGAA
Hairpin Sequence:
5'-CCGG-GCTGGCAGTAACTGGCAAGAA-CTCGAG-TTCTTGCCAGTTACTGCCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGCAGTAACTGGCAAGAACTCGAGTTCTTGCCAGTTACTGCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGCAGTAACTGGCAAGAACTCGAGTTCTTGCCAGTTACTGCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000275243