Construct: shRNA TRCN0000016672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_377878.1-143s1c1
DNA Barcode:
CCTCAGCTCCTCCTGCAGCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC402201 (402201)
Gene Description:
similar to Transcription initiation factor TFIID subunit 11 (Transcription initiation factor TFIID 28 kDa subunit) (TAF(II)28) (TAFII-28) (TAFII28) (TFIID subunit p30-beta)
Transcript:
RefSeq XM_377878.1 (NON-CURRENT)
Match location:
Position 143 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGCTCCTCCTGCAGCCA
Hairpin Sequence:
5'-CCGG-CCTCAGCTCCTCCTGCAGCCA-CTCGAG-TGGCTGCAGGAGGAGCTGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGCTCCTCCTGCAGCCACTCGAGTGGCTGCAGGAGGAGCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGCTCCTCCTGCAGCCACTCGAGTGGCTGCAGGAGGAGCTGAGG-3'

Other clones with same target sequence:

(none)