Construct: shRNA TRCN0000016739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_290732.3-3157s1c1
DNA Barcode:
GCAGAGGGAAATGATGTTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF157 (114804)
Gene Description:
ring finger protein 157
Transcript:
RefSeq XM_290732.3 (NON-CURRENT)
Match location:
Position 3157 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGGGAAATGATGTTATA
Hairpin Sequence:
5'-CCGG-GCAGAGGGAAATGATGTTATA-CTCGAG-TATAACATCATTTCCCTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGGGAAATGATGTTATACTCGAGTATAACATCATTTCCCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGGGAAATGATGTTATACTCGAGTATAACATCATTTCCCTCTGC-3'

Other clones with same target sequence:

(none)