Construct: shRNA TRCN0000016792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_060970.9-364s1c1
DNA Barcode:
CCAGATGTCTTCACCAGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRRXL1 (117065)
Gene Description:
paired related homeobox-like 1
Transcript:
RefSeq XM_060970.9 (NON-CURRENT)
Match location:
Position 364 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATGTCTTCACCAGAGAA
Hairpin Sequence:
5'-CCGG-CCAGATGTCTTCACCAGAGAA-CTCGAG-TTCTCTGGTGAAGACATCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATGTCTTCACCAGAGAACTCGAGTTCTCTGGTGAAGACATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATGTCTTCACCAGAGAACTCGAGTTCTCTGGTGAAGACATCTGG-3'

Other clones with same target sequence:

(none)