Construct: shRNA TRCN0000016805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020227.1-2004s1c1
DNA Barcode:
CGATAGGTCAAGCCTCTGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRDM9 (56979)
Gene Description:
PR/SET domain 9
Transcript:
RefSeq NM_020227.1 (NON-CURRENT)
Match location:
Position 2004 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATAGGTCAAGCCTCTGCTA
Hairpin Sequence:
5'-CCGG-CGATAGGTCAAGCCTCTGCTA-CTCGAG-TAGCAGAGGCTTGACCTATCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATAGGTCAAGCCTCTGCTACTCGAGTAGCAGAGGCTTGACCTATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATAGGTCAAGCCTCTGCTACTCGAGTAGCAGAGGCTTGACCTATCG-3'

Other clones with same target sequence:

(none)