Construct: shRNA TRCN0000016884

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144633.2-1023s1c1
DNA Barcode:
CCGAACAACTTATGTCAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNH8 (131096)
Gene Description:
potassium voltage-gated channel subfamily H member 8
Transcript:
RefSeq NM_144633.2 (NON-CURRENT)
Match location:
Position 1023 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016884)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAACAACTTATGTCAGCAA
Hairpin Sequence:
5'-CCGG-CCGAACAACTTATGTCAGCAA-CTCGAG-TTGCTGACATAAGTTGTTCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAACAACTTATGTCAGCAACTCGAGTTGCTGACATAAGTTGTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAACAACTTATGTCAGCAACTCGAGTTGCTGACATAAGTTGTTCGG-3'

Other clones with same target sequence:

(none)