Construct: shRNA TRCN0000016889

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020229.2-1584s1c1
DNA Barcode:
CCCTAACTGTATTCGCCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRDM11 (56981)
Gene Description:
PR/SET domain 11
Transcript:
RefSeq NM_020229.2 (NON-CURRENT)
Match location:
Position 1584 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016889)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAACTGTATTCGCCTAAA
Hairpin Sequence:
5'-CCGG-CCCTAACTGTATTCGCCTAAA-CTCGAG-TTTAGGCGAATACAGTTAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAACTGTATTCGCCTAAACTCGAGTTTAGGCGAATACAGTTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAACTGTATTCGCCTAAACTCGAGTTTAGGCGAATACAGTTAGGG-3'

Other clones with same target sequence:

(none)