Construct: shRNA TRCN0000016952

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032217.3-4258s1c1
DNA Barcode:
GCAGCAGATAACCGCAAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD17 (26057)
Gene Description:
ankyrin repeat domain 17
Transcript:
RefSeq NM_032217.3 (NON-CURRENT)
Match location:
Position 4258 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016952)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAGATAACCGCAAGATA
Hairpin Sequence:
5'-CCGG-GCAGCAGATAACCGCAAGATA-CTCGAG-TATCTTGCGGTTATCTGCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAGATAACCGCAAGATACTCGAGTATCTTGCGGTTATCTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAGATAACCGCAAGATACTCGAGTATCTTGCGGTTATCTGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000274424