Construct: shRNA TRCN0000016959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_064190.3-1869s1c1
DNA Barcode:
CGCTTTCCAAAGGAGGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSF5 (124535)
Gene Description:
heat shock transcription factor 5
Transcript:
RefSeq XM_064190.3 (NON-CURRENT)
Match location:
Position 1869 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTTCCAAAGGAGGAAGAA
Hairpin Sequence:
5'-CCGG-CGCTTTCCAAAGGAGGAAGAA-CTCGAG-TTCTTCCTCCTTTGGAAAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTTCCAAAGGAGGAAGAACTCGAGTTCTTCCTCCTTTGGAAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTTCCAAAGGAGGAAGAACTCGAGTTCTTCCTCCTTTGGAAAGCG-3'

Other clones with same target sequence:

(none)