Construct: shRNA TRCN0000016982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145657.1-477s1c1
DNA Barcode:
GCTGCCCAGCAGCAAGAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSX1 (219409)
Gene Description:
GS homeobox 1
Transcript:
RefSeq NM_145657.1 (NON-CURRENT)
Match location:
Position 477 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000016982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCCCAGCAGCAAGAGGAT
Hairpin Sequence:
5'-CCGG-GCTGCCCAGCAGCAAGAGGAT-CTCGAG-ATCCTCTTGCTGCTGGGCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCCCAGCAGCAAGAGGATCTCGAGATCCTCTTGCTGCTGGGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCCCAGCAGCAAGAGGATCTCGAGATCCTCTTGCTGCTGGGCAGC-3'

Other clones with same target sequence:

(none)