Construct: shRNA TRCN0000017000

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_069612.3-220s1c1
DNA Barcode:
GATCCCTTAGAGATACCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOBOX (135935)
Gene Description:
NOBOX oogenesis homeobox
Transcript:
RefSeq XM_069612.3 (NON-CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017000)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCCTTAGAGATACCTGAA
Hairpin Sequence:
5'-CCGG-GATCCCTTAGAGATACCTGAA-CTCGAG-TTCAGGTATCTCTAAGGGATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCCTTAGAGATACCTGAACTCGAGTTCAGGTATCTCTAAGGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCCTTAGAGATACCTGAACTCGAGTTCAGGTATCTCTAAGGGATC-3'

Other clones with same target sequence:

(none)