Construct: shRNA TRCN0000017093

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016604.2-1628s1c1
DNA Barcode:
CCCTAGTTCATCGCAACCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KDM3B (51780)
Gene Description:
lysine demethylase 3B
Transcript:
RefSeq NM_016604.2 (NON-CURRENT)
Match location:
Position 1628 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017093)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAGTTCATCGCAACCTTT
Hairpin Sequence:
5'-CCGG-CCCTAGTTCATCGCAACCTTT-CTCGAG-AAAGGTTGCGATGAACTAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAGTTCATCGCAACCTTTCTCGAGAAAGGTTGCGATGAACTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAGTTCATCGCAACCTTTCTCGAGAAAGGTTGCGATGAACTAGGG-3'

Other clones with same target sequence:

  1. TRCN0000274391