Construct: shRNA TRCN0000017124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003086.1-3784s1c1
DNA Barcode:
CCAGCCAATATGAACAGGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNAPC4 (6621)
Gene Description:
small nuclear RNA activating complex polypeptide 4
Transcript:
RefSeq NM_003086.1 (NON-CURRENT)
Match location:
Position 3784 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCAATATGAACAGGGAA
Hairpin Sequence:
5'-CCGG-CCAGCCAATATGAACAGGGAA-CTCGAG-TTCCCTGTTCATATTGGCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCAATATGAACAGGGAACTCGAGTTCCCTGTTCATATTGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCAATATGAACAGGGAACTCGAGTTCCCTGTTCATATTGGCTGG-3'

Other clones with same target sequence:

(none)