Construct: shRNA TRCN0000017154

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002509.2-679s1c1
DNA Barcode:
GCCGACGAGTCACCGGACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NKX2-2 (4821)
Gene Description:
NK2 homeobox 2
Transcript:
RefSeq NM_002509.2 (NON-CURRENT)
Match location:
Position 679 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017154)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGACGAGTCACCGGACAAT
Hairpin Sequence:
5'-CCGG-GCCGACGAGTCACCGGACAAT-CTCGAG-ATTGTCCGGTGACTCGTCGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGACGAGTCACCGGACAATCTCGAGATTGTCCGGTGACTCGTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGACGAGTCACCGGACAATCTCGAGATTGTCCGGTGACTCGTCGGC-3'

Other clones with same target sequence:

(none)