Construct: shRNA TRCN0000017168

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_377946.1-843s1c1
DNA Barcode:
GCTGCTCTTCAACACAAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC402280 (402280)
Gene Description:
similar to GTF2I repeat domain containing 1 isoform 2; Williams-Beuren syndrome chromosome region 11; GTF2I repeat domain-containing 1; general transcription factor 3; muscle TFII-I repeat domain-containing protein 1 alpha 1
Transcript:
RefSeq XM_377946.1 (NON-CURRENT)
Match location:
Position 843 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017168)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTCTTCAACACAAGATA
Hairpin Sequence:
5'-CCGG-GCTGCTCTTCAACACAAGATA-CTCGAG-TATCTTGTGTTGAAGAGCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTCTTCAACACAAGATACTCGAGTATCTTGTGTTGAAGAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTCTTCAACACAAGATACTCGAGTATCTTGTGTTGAAGAGCAGC-3'

Other clones with same target sequence:

(none)