Construct: shRNA TRCN0000017172
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_377946.1-388s1c1
- DNA Barcode:
- GTAAGCTACAAGGAGGAGCTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LOC402280 (402280)
- Gene Description:
- similar to GTF2I repeat domain containing 1 isoform 2; Williams-Beuren syndrome chromosome region 11; GTF2I repeat domain-containing 1; general transcription factor 3; muscle TFII-I repeat domain-containing protein 1 alpha 1
- Transcript:
- RefSeq XM_377946.1 (NON-CURRENT)
- Match location:
- Position 388 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000017172)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTAAGCTACAAGGAGGAGCTT
- Hairpin Sequence:
-
5'-CCGG-GTAAGCTACAAGGAGGAGCTT-CTCGAG-AAGCTCCTCCTTGTAGCTTAC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTAAGCTACAAGGAGGAGCTTCTCGAGAAGCTCCTCCTTGTAGCTTACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTAAGCTACAAGGAGGAGCTTCTCGAGAAGCTCCTCCTTGTAGCTTAC-3'
Other clones with same target sequence:
(none)
