Construct: shRNA TRCN0000017213

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177398.1-1400s1c1
DNA Barcode:
CCCATATGGAACAACCATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LMX1A (4009)
Gene Description:
LIM homeobox transcription factor 1 alpha
Transcript:
RefSeq NM_177398.1 (NON-CURRENT)
Match location:
Position 1400 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017213)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATATGGAACAACCATATT
Hairpin Sequence:
5'-CCGG-CCCATATGGAACAACCATATT-CTCGAG-AATATGGTTGTTCCATATGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATATGGAACAACCATATTCTCGAGAATATGGTTGTTCCATATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATATGGAACAACCATATTCTCGAGAATATGGTTGTTCCATATGGG-3'

Other clones with same target sequence:

(none)