Construct: shRNA TRCN0000017216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177398.1-702s1c1
DNA Barcode:
GCAGCCTCAGACTCAGGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LMX1A (4009)
Gene Description:
LIM homeobox transcription factor 1 alpha
Transcript:
RefSeq NM_177398.1 (NON-CURRENT)
Match location:
Position 702 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCTCAGACTCAGGTAAA
Hairpin Sequence:
5'-CCGG-GCAGCCTCAGACTCAGGTAAA-CTCGAG-TTTACCTGAGTCTGAGGCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCTCAGACTCAGGTAAACTCGAGTTTACCTGAGTCTGAGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCTCAGACTCAGGTAAACTCGAGTTTACCTGAGTCTGAGGCTGC-3'

Other clones with same target sequence:

(none)