Construct: shRNA TRCN0000017222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003425.2-682s1c1
DNA Barcode:
GCAACAGATTACAAGAGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF45 (7596)
Gene Description:
zinc finger protein 45
Transcript:
RefSeq NM_003425.2 (NON-CURRENT)
Match location:
Position 682 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACAGATTACAAGAGAGTT
Hairpin Sequence:
5'-CCGG-GCAACAGATTACAAGAGAGTT-CTCGAG-AACTCTCTTGTAATCTGTTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACAGATTACAAGAGAGTTCTCGAGAACTCTCTTGTAATCTGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACAGATTACAAGAGAGTTCTCGAGAACTCTCTTGTAATCTGTTGC-3'

Other clones with same target sequence:

  1. TRCN0000280434