Construct: shRNA TRCN0000017243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001533.1-749s1c1
DNA Barcode:
CGCTTGAATGTGTTCAAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HNRNPL (3191)
Gene Description:
heterogeneous nuclear ribonucleoprotein L
Transcript:
RefSeq NM_001533.1 (NON-CURRENT)
Match location:
Position 749 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTGAATGTGTTCAAGAAT
Hairpin Sequence:
5'-CCGG-CGCTTGAATGTGTTCAAGAAT-CTCGAG-ATTCTTGAACACATTCAAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTGAATGTGTTCAAGAATCTCGAGATTCTTGAACACATTCAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTGAATGTGTTCAAGAATCTCGAGATTCTTGAACACATTCAAGCG-3'

Other clones with same target sequence:

  1. TRCN0000330620