Construct: shRNA TRCN0000017336

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006162.3-810s1c1
DNA Barcode:
CATCGAGATAACCTCGTGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NFATC1 (4772)
Gene Description:
nuclear factor of activated T cells 1
Transcript:
RefSeq NM_006162.3 (NON-CURRENT)
Match location:
Position 810 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017336)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGAGATAACCTCGTGCTT
Hairpin Sequence:
5'-CCGG-CATCGAGATAACCTCGTGCTT-CTCGAG-AAGCACGAGGTTATCTCGATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGAGATAACCTCGTGCTTCTCGAGAAGCACGAGGTTATCTCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGAGATAACCTCGTGCTTCTCGAGAAGCACGAGGTTATCTCGATG-3'

Other clones with same target sequence:

(none)