Construct: shRNA TRCN0000017396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_086344.5-997s1c1
DNA Barcode:
GCACACCAGCTGTTATATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC148864 (148864)
Gene Description:
similar to LIM homeo domain transcription factor
Transcript:
RefSeq XM_086344.5 (NON-CURRENT)
Match location:
Position 997 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACCAGCTGTTATATTAA
Hairpin Sequence:
5'-CCGG-GCACACCAGCTGTTATATTAA-CTCGAG-TTAATATAACAGCTGGTGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACCAGCTGTTATATTAACTCGAGTTAATATAACAGCTGGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACCAGCTGTTATATTAACTCGAGTTAATATAACAGCTGGTGTGC-3'

Other clones with same target sequence:

(none)