Construct: shRNA TRCN0000017416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020338.1-2634s1c1
DNA Barcode:
GCCCAATGTCATGGAGATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZMIZ1 (57178)
Gene Description:
zinc finger MIZ-type containing 1
Transcript:
RefSeq NM_020338.1 (NON-CURRENT)
Match location:
Position 2634 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAATGTCATGGAGATGAT
Hairpin Sequence:
5'-CCGG-GCCCAATGTCATGGAGATGAT-CTCGAG-ATCATCTCCATGACATTGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAATGTCATGGAGATGATCTCGAGATCATCTCCATGACATTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAATGTCATGGAGATGATCTCGAGATCATCTCCATGACATTGGGC-3'

Other clones with same target sequence:

(none)