Construct: shRNA TRCN0000017454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203394.1-776s1c1
DNA Barcode:
CGCATCTATGACATTGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
E2F7 (144455)
Gene Description:
E2F transcription factor 7
Transcript:
RefSeq NM_203394.1 (NON-CURRENT)
Match location:
Position 776 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATCTATGACATTGTAAAT
Hairpin Sequence:
5'-CCGG-CGCATCTATGACATTGTAAAT-CTCGAG-ATTTACAATGTCATAGATGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATCTATGACATTGTAAATCTCGAGATTTACAATGTCATAGATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATCTATGACATTGTAAATCTCGAGATTTACAATGTCATAGATGCG-3'

Other clones with same target sequence:

(none)