Construct: shRNA TRCN0000017517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002316.1-378s1c1
DNA Barcode:
GCTACGCAAGGGCGACGAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LMX1B (4010)
Gene Description:
LIM homeobox transcription factor 1 beta
Transcript:
RefSeq NM_002316.1 (NON-CURRENT)
Match location:
Position 378 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACGCAAGGGCGACGAATT
Hairpin Sequence:
5'-CCGG-GCTACGCAAGGGCGACGAATT-CTCGAG-AATTCGTCGCCCTTGCGTAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACGCAAGGGCGACGAATTCTCGAGAATTCGTCGCCCTTGCGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACGCAAGGGCGACGAATTCTCGAGAATTCGTCGCCCTTGCGTAGC-3'

Other clones with same target sequence:

(none)