Construct: shRNA TRCN0000017538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005583.3-1258s1c1
DNA Barcode:
CGTCAAGGAAAGGGCAGTGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYL1 (4066)
Gene Description:
LYL1 basic helix-loop-helix family member
Transcript:
RefSeq NM_005583.3 (NON-CURRENT)
Match location:
Position 1258 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCAAGGAAAGGGCAGTGGA
Hairpin Sequence:
5'-CCGG-CGTCAAGGAAAGGGCAGTGGA-CTCGAG-TCCACTGCCCTTTCCTTGACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCAAGGAAAGGGCAGTGGACTCGAGTCCACTGCCCTTTCCTTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCAAGGAAAGGGCAGTGGACTCGAGTCCACTGCCCTTTCCTTGACG-3'

Other clones with same target sequence:

(none)