Construct: shRNA TRCN0000017598

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013434.3-599s1c1
DNA Barcode:
GCCTTTAATCTCTACGACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNIP3 (30818)
Gene Description:
potassium voltage-gated channel interacting protein 3
Transcript:
RefSeq NM_013434.3 (NON-CURRENT)
Match location:
Position 599 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017598)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTAATCTCTACGACATT
Hairpin Sequence:
5'-CCGG-GCCTTTAATCTCTACGACATT-CTCGAG-AATGTCGTAGAGATTAAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTAATCTCTACGACATTCTCGAGAATGTCGTAGAGATTAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTAATCTCTACGACATTCTCGAGAATGTCGTAGAGATTAAAGGC-3'

Other clones with same target sequence:

(none)