Construct: shRNA TRCN0000017678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005461.3-2519s1c1
DNA Barcode:
GCCTTGTCTTATGGTCAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAFB (9935)
Gene Description:
MAF bZIP transcription factor B
Transcript:
RefSeq NM_005461.3 (NON-CURRENT)
Match location:
Position 2519 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGTCTTATGGTCAAATT
Hairpin Sequence:
5'-CCGG-GCCTTGTCTTATGGTCAAATT-CTCGAG-AATTTGACCATAAGACAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGTCTTATGGTCAAATTCTCGAGAATTTGACCATAAGACAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGTCTTATGGTCAAATTCTCGAGAATTTGACCATAAGACAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000274269