Construct: shRNA TRCN0000017703

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014552.2-1791s1c1
DNA Barcode:
CCCATCTTTGAAGGGCTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRHL1 (29841)
Gene Description:
grainyhead like transcription factor 1
Transcript:
RefSeq NM_014552.2 (NON-CURRENT)
Match location:
Position 1791 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017703)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCTTTGAAGGGCTTGAT
Hairpin Sequence:
5'-CCGG-CCCATCTTTGAAGGGCTTGAT-CTCGAG-ATCAAGCCCTTCAAAGATGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCTTTGAAGGGCTTGATCTCGAGATCAAGCCCTTCAAAGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCTTTGAAGGGCTTGATCTCGAGATCAAGCCCTTCAAAGATGGG-3'

Other clones with same target sequence:

  1. TRCN0000329980