Construct: shRNA TRCN0000017757

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024827.1-964s1c1
DNA Barcode:
GACTCCATACTTAATCTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HDAC11 (79885)
Gene Description:
histone deacetylase 11
Transcript:
RefSeq NM_024827.1 (NON-CURRENT)
Match location:
Position 964 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017757)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCCATACTTAATCTGTTT
Hairpin Sequence:
5'-CCGG-GACTCCATACTTAATCTGTTT-CTCGAG-AAACAGATTAAGTATGGAGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCCATACTTAATCTGTTTCTCGAGAAACAGATTAAGTATGGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCCATACTTAATCTGTTTCTCGAGAAACAGATTAAGTATGGAGTC-3'

Other clones with same target sequence:

  1. TRCN0000330866