Construct: shRNA TRCN0000017806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199262.2-581s1c1
DNA Barcode:
ACACCATTATGAATCGTGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SP6 (80320)
Gene Description:
Sp6 transcription factor
Transcript:
RefSeq NM_199262.2 (NON-CURRENT)
Match location:
Position 581 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACCATTATGAATCGTGGTT
Hairpin Sequence:
5'-CCGG-ACACCATTATGAATCGTGGTT-CTCGAG-AACCACGATTCATAATGGTGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACCATTATGAATCGTGGTTCTCGAGAACCACGATTCATAATGGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACCATTATGAATCGTGGTTCTCGAGAACCACGATTCATAATGGTGT-3'

Other clones with same target sequence:

(none)