Construct: shRNA TRCN0000017861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030767.2-2320s1c1
DNA Barcode:
CCCTGCCCATTGCACGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKNA (80709)
Gene Description:
AT-hook transcription factor
Transcript:
RefSeq NM_030767.2 (NON-CURRENT)
Match location:
Position 2320 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGCCCATTGCACGTAAAT
Hairpin Sequence:
5'-CCGG-CCCTGCCCATTGCACGTAAAT-CTCGAG-ATTTACGTGCAATGGGCAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGCCCATTGCACGTAAATCTCGAGATTTACGTGCAATGGGCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGCCCATTGCACGTAAATCTCGAGATTTACGTGCAATGGGCAGGG-3'

Other clones with same target sequence:

(none)