Construct: shRNA TRCN0000017867

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_095746.8-465s1c1
DNA Barcode:
GCAGAACAGCATCCGCCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXD4 (2298)
Gene Description:
forkhead box D4
Transcript:
RefSeq XM_095746.8 (NON-CURRENT)
Match location:
Position 465 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017867)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAACAGCATCCGCCACAA
Hairpin Sequence:
5'-CCGG-GCAGAACAGCATCCGCCACAA-CTCGAG-TTGTGGCGGATGCTGTTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAACAGCATCCGCCACAACTCGAGTTGTGGCGGATGCTGTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAACAGCATCCGCCACAACTCGAGTTGTGGCGGATGCTGTTCTGC-3'

Other clones with same target sequence:

(none)