Construct: shRNA TRCN0000017909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012081.3-1254s1c1
DNA Barcode:
GCCGTTCAGAATCTCCTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELL2 (22936)
Gene Description:
elongation factor for RNA polymerase II 2
Transcript:
RefSeq NM_012081.3 (NON-CURRENT)
Match location:
Position 1254 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000017909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTTCAGAATCTCCTGTAT
Hairpin Sequence:
5'-CCGG-GCCGTTCAGAATCTCCTGTAT-CTCGAG-ATACAGGAGATTCTGAACGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTTCAGAATCTCCTGTATCTCGAGATACAGGAGATTCTGAACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTTCAGAATCTCCTGTATCTCGAGATACAGGAGATTCTGAACGGC-3'

Other clones with same target sequence:

(none)